A Home Dna Kit For Paternity Testing And The Cost Of A Paternity Test

Many people are uncertain to undergo the paternity testing process. One reason can be the cost of paternity test, which would be merely expensive. However, it is worth it once you obtain the test results.  Paternity testing kits are the best method to perform an assurance test.

Many reasons exist on why people desire to undergo paternity DNA testing. One reason is reassurance. Getting a paternity test is a good idea, if an individual has more than one partner at the conception time. There are also women, who never think about the cost of paternity test. However, they get this test done to get their child support.

Generally , the court  may  order the father as well , to undergo  the paternity test , which would definitely  guarantee  that the  child belongs to the alleged father, in regard to the child’s financial responsibility . The cost of paternity testing can be quite high for multiple potential fathers.

There are multiple ways in order to undertake paternity testing. One type of paternity test is simple by taking DNA samples from the cheeks of both the child and potential father. This test can be done at your own using DNA home paternity testing kits. The third type of paternity test is done before the child is born. But , for this , you have to  consult the doctor  initially  and the  procedure  is quite a delicate  one, as doctors  will take amniotic fluid that contains  DNA from the uterus. This process may harm the baby and so it is best to choose a home DNA kit.

The total cost of a paternity test typically ranges from $400 to $2,000 for legal cases. However, Home DNA kits are available, which are affordable and easy to carry out.

You can get results usually within a week and it depends on the laboratory that administers it. It is recommended to look at your options when you think about this matter.

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The Amelogenin Sex Gene in Dna Paternity Testing

DNA testing is now the most widely used method for determining the biological relationships between individuals. The DNA paternity test is probably the most widely known of these tests and involves the testing of an alleged father, mother and the child in question. This test can also be conducted just between Alleged Father and a child without the Mothers sample.

When performing the Paternity Test, most DNA testing laboratories will test a minimum of 16 genetic markers for enhanced accuracy levels in excess of 99.99%. One of these genes tested is called the ‘Amelogenin’ gene and its use is to determine the sex of the sample that has been processed. Therefore in reality the statistical analysis is actually based on the matching of 15 of these 16 markers.

From a biological perspective, males have an XY chromosome and females have an XX chromosome. Therefore in your DNA test result you should see an XY for the alleged father and the child if he is male, and XX for the Mother and child if she is female. A DNA paternity test result from any serious service provider should report this gene together with the rest of the genetic markers analysed.

Functions and Uses of Testing the Amelogenin Gene

The testing of this gene may appear superfluous to a client at first instance, however it does serve a number of functions.

1. From a laboratory perspective, it acts as a primary quality control check and ensures that the correct samples have been processed. So for example, if the Alleged Father’s sample turns out to be female, then there is an element of quality control to identify the possible reasons. These could include problems such as the client putting the wrong samples in the wrong envelopes (e.g. putting the father’s sample in the mother’s envelope and vice versa). The testing of this gene can also act as a deterrent for submitting someone else’s samples (having said that, this applies only if the sample of someone of the opposite sex is supplied).

2. Gender identification through DNA testing has also got applications in the field of forensic DNA testing as it provides primary information regarding samples found at the scene of the crime during a criminal investigation. Through the test, it is possible to confirm whether biological remains found at the crime scene belong to males or females. It is also possible to differentiate this even in cases of mixed samples.

3. Gender identification is also applicable in the case of infidelity DNA testing. Analysis of the samples will show whether suspicious stains belong to a male or female individual. On the basis of this result, decisions for further testing can be based.

However, gender identification by amplification of the amelogenin gene can sometimes generate a result in which male samples were falsely identified as females. I have myself only seen few of these cases over the years. This occurrence is therefore very rare and occurs in less than 0.01% of cases in males and even less in females according to some studies. In such cases, it is suggested to perform the Y amplification of SRY gene or/and Y-STR markers to confirm the gender

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Paternity Testing on Absent Fathers

DNA testing is one of the most conclusive ways available to determine paternity of any given child. By taking a sample of DNA from both the alleged father and from the child in question, high-tech DNA profiling techniques are used to match reference points across genetic markers on both samples which can lead to an accurate determination of where a genetic relationship exists.

In an ideal world, paternity testing would have available both a sample from the child and from his alleged father in order to best establish the link between them. However, in some DNA paternity testing cases the father is not available to give his DNA sample, whether through choice, or unavailability for some other reason. However, where the father is absent, it is still possible to use other DNA tests to determine whether a relationship exists.

DNA Testing on Other Direct Relatives

By performing DNA testing with other members of the family that share some of the same genes with the alleged father, it is possible to determine whether or not there is a likelihood of paternity over the child in question. Obviously the father’s sample is desired for reasons of accuracy and conclusivity, however it is more than possible (particularly with advancing testing technologies) to be able to determine paternity through shared genetic material through more distant relatives to the father. In other words, provided a relative of the alleged father is available for DNA testing, you should be able to obtain some results although this may not always be conclusive.

DNA Testing on Grandparents

In the absence of the alleged father or any separate sample of his DNA, the alleged paternal grandparents can provide the key to establishing paternity. By taking a sample from either one or both the grandmother and grandfather of the baby, it is possible to determine even in the absence of the father whether or not there is shared genetic material and consequently whether or not there is a shared relationship, i.e. parentage.

DNA Testing on Male Relatives

Y-STR testing is one of the most innovative DNA testing methods honed in recent years, tracking down the male-only Y- chromosome to establish genetic links between male children and elder vertical male relatives, for example grandfathers, great grandfathers, even great uncles by blood relationships. This can be particularly handy where the father is unavailable and direct elderly relatives have died, given that a great deal of genetic material passes unmutated from father to son throughout generations. Of course, this is however absolutely of no use for DNA testing of female children.

DNA Testing on Siblings

Alternatively siblings can also provide a close genetic match, which makes it possible to test whether or not two children are related by the whole blood, rather than half. Where there is dispute over the potential father of a child, an alleged sibling’s sample can be a good way to compare DNA to determine the extent of the relationship. The same can be said for brothers and sisters of the alleged father, who can also share some genetic material sufficient to provide a result in DNA testing of the child in question,

If your situation requires is such that you require to do the DNA testing through one of the alternatives above, it is always advisable to contact in advance the company who will be performing the DNA test and explain your case in full so that they can advise you on the DNA testing options available as well the results that can be obtained.

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Five Top Things to Know About a Dna Paternity Test

Whilst the awareness about DNA Paternity testing has greatly increased throughout most of the world, there is still a lot of information about the test that is not known.

The following are the five top things to know about a DNA Paternity Test:

1. Most accurate method of determining biological relationships

A DNA Paternity test is the most accurate way of determining a biological relationship between an alleged father/mother and their child. Whilst in the past blood grouping analysis was used as a screening method, the relevance of this test has been greatly reduced as the level of accuracy of this type of test is low compared to the result of a DNA test. A DNA paternity test will provide 100% certainty of exclusion and 99.9%+ probability of inclusion.

2. Difference between an At-Home Paternity Test and a Legal Paternity Test

The difference between an At-Home Paternity Test (also called Curiosity Test) and a Legal Paternity Test is not the actual result but the way in which the sample collection is performed. For an At-Home Paternity test, a home dna kit is normally sent to the requesting party. The kit will normally include i) oral swabs to take the saliva sample, ii) documentation to provide relevant information including consent iii) instructions and terms and conditions of sale. The sample is taken by the parties themselves who are participating in the test. In a Legal DNA Paternity Test, the sample collection must follow a strict chain of custody procedure. All samples need to be collected and documented by a independent third party (normally a qualified general practitioner or nurse) who will be responsible to verify and authenticate the samples. The actual result of the paternity in terms of the biological relationship will not differ.

3. Always select a properly accredited laboratory

Always chose to do your paternity test through a company that uses an ISO17025 and/or AABB accredited laboratory. This will ensure the quality and accuracy of the results. Also make sure that when report the result a full DNA profile is provided for the 16 loci analysed, as well as the statistical probability of paternity. A simple Yes/No answer should not be deemed acceptable.

4. Paternity tests can be performed without the sample of the mother

To perform a DNA Paternity test the sample of the Mother is not necessary to obtain an accurate result. However, when available, it is always recommended that the sample of the Mother is also provided. Only in specific cases will the sample of the mother provide more accurate results such in the case of gene mutation. Many companies offer to test the mother at no extra charge.

5. DNA can be obtained from a diverse range of samples

It is possible to obtain DNA from a number of other sources such as hair, blood, semen and items such as cigarette butts and a toothbrush. Whilst the current standard is to use an oral swab to collect saliva samples, in cases where a person may not be physically present, able or willing to donate a sample, then alternate samples can be obtained.

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Dna Paternity Testing of Related Potential Fathers

DNA paternity testing of related potential fathers is not uncommon, although paternity in such cases is more difficult to prove beyond doubt. It is frequently possible if the relationship is known prior to testing, though in some such cases other evidence is generally required before paternity can be legally ascertained.

While positive DNA tests can be accepted as positive evidence of paternity in the vast majority of cases due the statistical improbability of potential fathers having the same DNA profiles, the situation is complicated if the possible fathers are related. It isn’t uncommon for brothers to be involved in a parentage dispute, or even for the mother to have the DNA test carried out privately to determine which is the father of her child.

In normal paternity disputes, whether or not DNA profiling is acceptable as the sole evidence depends upon the circumstances of the case, and it is generally wise to be able to prove the likelihood, or even just the possibility, of the mother and the possible father coming into contact with one another. In Islamic law a paternity test is not sufficient, and other evidence, preferably that of witnesses, is necessary.

The Legal Aspects

Where DNA paternity testing is to be used in evidence, courts will generally insist on a legal paternity test where the collection of the sampling is carried out by an authorized professional and the identity of the subject legally determined. Home DNA testing, or DNA tests on samples presented by the subject, is not admissible as evidence. The probability of paternity accepted as definitive proof varies from state to state, although a probability of 99% would seem reasonable where there is no doubt as to the source of the sample, and where no complications such as rape are involved.

The fact that parentage has been discounted in 99 men in 100 is a more positive identification of the father where there are limited possibilities than were the father potentially any individual in the general population such as occurs with rape. In that case a probability of 99.9% or higher would be more definite, together with additional proof of the likelihood of the suspect being in the vicinity.

Such probability is determined largely by the accuracy of the test that depends upon both the way it is carried out and the number of DNA indicators used (a good average would be 16), and is frequently as high as 99.99% or more. It also depends on whether the sample of the Mother is included in the testing or not, as this will help to obtain a much higher level of probability.

Common DNA between Family Members

DNA tests on related potential fathers are no different from those carried out on any other subject: it is the results that could potentially create problems. Take the case of first cousins: they share 12.5% of their DNA and so it will be more difficult to prove parentage. This sharing of DNA increases to 25% for uncles, nephews and half-brothers, and to 50% for sons, fathers and brothers of the person being tested.

DNA paternity testing on different members of the same family is not an uncommon situation, and can occur for a number of reasons, including:

a) Parentage of pregnancies arising from consensual and non-consensual relationships, although the former is by far the more common of the two.
b) Two family members each claiming parentage, whether or not the mother is claiming one or neither to be the father.
c) A brother desiring confirmation that he is the parent.
d) The mother, or potential mother, having a private test to assure herself as to who is the father.
e) A grown child confirming parentage, particularly where an inheritance could be involved.

There are others, but these are common reasons for legal and home DNA tests being carried out where potential fathers could be related. Standard DNA paternity testing statistics are based upon unrelated members of the public, and unless the laboratory carrying out the DNA testing has been informed of the relationship, the test results could be misinterpreted.

The Case of Identical Twins

Nevertheless, it is possible to test additional DNA markers if any of the above relationships are known, but not in one specific case. This is the case of identical twins. Normal twins occur when two eggs are fertilized by two sperm, and are no more alike genetically than were they siblings born years apart. Identical twins, however, are formed when one egg is fertilized by one sperm and then splits into two halves within the first 14 days for no known reason.

These twins share an identical genetic make-up and so their DNA is identical and indistinguishable from one another. There is no test that can determine which is the biological father of a child. Unless one is excluded for some reason, either could be the father and that is all that DNA testing can prove. The court would have to disregard DNA results and use other means to determine paternity.

Old-fashioned policing, therefore, still has a part to play where the related potential fathers are identical twins. Apart from this specific case, DNA paternity tests are not entirely foolproof since they rely on probability, but are virtually conclusive where you simply want confirmation of paternity. In such a case, a likelihood of 1 in a 1000 is a virtual certainty and where confirmation is required between a limited number of candidates, a 99.9% DNA genetic testing result is legal confirmation.

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